- Fecal Incontinence
|Mitochondrial dysfunction in inflammatory bowel disease alters intestinal epithelial metabolism of hepatic acylcarnitines
J Clin Invest. 2020 Nov 3;133371.doi: 10.1172/JCI133371. Online ahead of print.
Sarah A Smith 1, Sayaka A Ogawa 1, Lillian Chau 1, Kelly A Whelan 1, Kathryn E Hamilton 2, Jie Chen 3, Lu Tan 3, Eric Z Chen 4, Sue Keilbaugh 1, Franz Fogt 5, Meenakshi Bewtra 1, Jonathan Braun 6, Ramnik J Xavier 7, Clary B Clish 8, Barry Slaff 9, Aalim M Weljie 9, Frederic D Bushman 10, James D Lewis 1, Hongzhe Li 11, Stephen R Master 3, Michael J Bennett 3, Hiroshi Nakagawa 12, Gary D Wu 1
As the interface between the gut microbiota and the mucosal immune system, there has been great interest in the maintenance of colonic epithelial integrity through mitochondrial oxidation of butyrate, a short-chain fatty acid produced by the gut microbiota. Herein, we showed that the intestinal epithelium can also oxidize long-chain fatty acids, and that luminally-delivered acylcarnitines in bile can be consumed via apical absorption by the intestinal epithelium resulting in mitochondrial oxidation. Finally, intestinal inflammation led to mitochondrial dysfunction in the apical domain of the surface epithelium that may reduce the consumption of fatty acids, contributing to higher concentrations of fecal acylcarnitines in murine Citrobacter rodentium-induced colitis and human inflammatory bowel disease. These results emphasized the importance of both the gut microbiota and the liver in the delivery of energy substrates for mitochondrial metabolism by the intestinal epithelium.