Abstract

Variation of Gut Mucosal Microbiome with ASCA Status in Pediatric Crohn

Kansal S1,2,3, Catto-Smith AG3,4, Boniface K2, Thomas S2, Cameron DJ1, Oliver M1, Alex G1, Kirkwood CD2,5, Wagner J3,6. J Pediatr Gastroenterol Nutr. 2019 Aug 6. doi: 10.1097/MPG.0000000000002461. [Epub ahead of print]

 
     

Author information

Department of Gastroenterology and Clinical Nutrition, Royal Children's Hospital, Parkville, Victoria, Australia.

Enteric Virus group, Murdoch Children's Research Institute, Parkville, Victoria, Australia.

Department of Pediatrics, University of Melbourne, Parkville, Victoria, Australia.

Department of Gastroenterology, Hepatology and Liver Transplant, Lady Cilento Children's Hospital, Brisbane, Queensland, Australia.

Enteric and Diarrheal Diseases, Global Health, Bill and Melinda Gates Foundation, Seattle, Washington, USA.

Wellcome Trust, Sanger Institute, Cambridge, UK.

Abstract

OBJECTIVES: Crohn's Disease (CD) is a chronic relapsing condition possibly caused by a dysbiotic microbiome. About 30-60% of CD patients have antibodies to Saccharomyces cerevisiae (ASCA), but any association with gut microbiota is unexplored. We hypothesized that ASCA positivity would predict a signature microbial status and clinical phenotype.

METHODS: Ileocolonic mucosal biopsies were obtained from children with CD (n?=?135), and controls without inflammatory boweldisease (n?=?45). Comparison was made between ASCA status, microbial diversity and clinical characteristics.

RESULTS: ASCA was highly specific but poorly sensitive for the diagnosis of CD. In patients with CD, ASCA positivity was associated with older age (≥10 years), ileocolonic disease and long-term risk of surgery. Microbial alpha and beta diversity were similar in CD patients with or without ASCA, but significantly less when compared to non-IBD controls. Microbial richness was similar across all three groups. Fourteen bacterial species were associated with ASCA positive CD patients and 14 species with ASCA negative patients (p?

CONCLUSION: ASCA positive and ASCA negative CD patients have significant differences in gut microbiome composition, which could possibly be influencing the phenotype of the disease.

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