Abstract

Evaluation of Escherichia coli pathotypes associated with irritable bowel syndrome

Dogan B1, Belcher-Timme HF1, Dogan EI1, Jiang ZD2, DuPont HL2,3, Snyder N3, Yang S1, Chandler B1, Scherl EJ4, Simpson KW1. FEMS Microbiol Lett. 2018 Nov 1;365(22). doi: 10.1093/femsle/fny249.
 
     

Author information

1 Department of Clinical Sciences, Cornell University College of Veterinary Medicine, 930 Campus Road, Ithaca, NY 14853, USA.

2 University of Texas Health Science Center at Houston, School of Public Health, 1200 Pressler Street, Houston, TX 77030, USA.

3 Kelsey Research Foundation, 5615 Kirby Drive, 77005 and the Kelsey Seybold Clinic, 2727 West Holcombe Blvd., 77025, Houston, TX, USA.

4 Jill Roberts Center for Inflammatory Bowel Disease, New York-Presbyterian Hospital, Weill Cornell Medical Center, 1283 York Avenue, New York, NY 10065.

Abstract

Irritable bowel syndrome (IBS) affects 10%-20% of people. Increased numbers of Escherichia coli (E. coli) correlate with symptoms, and patients respond to antimicrobials targeting E. coli. We examined whether specific E. coli strains, phylogroups and pathotypes are associated with IBS. We evaluated 218 E. coli isolates from 33 IBS patients and 23 healthy controls. RAPD analysis revealed 89 E. coli strains (29 controls, 60 IBS), spanning the A, B1, B2 and D phylogroups. Strains were similarly enriched in virulence genes associated with extraintestinal pathogenic E. coli (ExPEC) and/or adherent-invasive E. coli (AIEC). Three strains harbored a diarrheagenic virulence gene (2 IBS, 1 control). Escherichia coli capable of invading epithelial cells or replicating in macrophages were detected in 53% of IBS and 50% controls, and 67% IBS and 45% controls respectively (P > 0.05). AIEC were identified in 33% of IBS patients vs 20% of controls (P = 0.35). Virulence genes ibeA, ColV and pduC were associated with intramacrophage persistence; ibeA and ColV were associated with epithelial invasion and AIEC pathotype (P < 0.05). IBS patients and controls are commonly colonized by E. coli that resemble ExPEC and display pathogen-like behavior in vitro, similar to CD-associated AIEC. The relationship of these resident pathosymbiont E. coli to IBS warrants further investigation.

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